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Title: | Real-time interrogation of fiber optic biosensor using TiO2coated etched long-period grating |
Authors: | Singh M. Raghuwanshi S.K. |
Issue Date: | 2020 |
Citation: | Review of Scientific Instruments Vol. 91 , 12 , p. - |
Abstract: | In this work, a TiO2 coated etched long-period grating (e-LPG) fiber optic biosensor is developed for the detection of Escherichia coli (E. coli) bacteria in food items. Label-free Escherichia coli bacteria monitoring is done over the detection range of 0 cfu/ml-50 cfu/ml using an advanced spectral interrogation mechanism. The thin film deposition of 40 nm TiO2 over the e-LPG is confirmed by the microscopy method, such as scanning electron microscopy. In our proposed biosensor design, T4-bacteriophage is covalently immobilized over the TiO2 coated fiber surface. This biosensor system has reached sensitivity at 2.55 nm/RIU. Our experiments confirm the resolution and the limit of detection (3σ/S) of 0.0039 RIU and 10.05 ppm, respectively. The proposed biosensor with enhanced sensitivity is suitable for monitoring harmful pathogens/infectious agents in various food products. © 2020 Author(s). |
URI: | https://doi.org/10.1063/5.0020571 http://idr.nitk.ac.in/jspui/handle/123456789/15782 |
Appears in Collections: | 1. Journal Articles |
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